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TG-14 using PrimeSTAR HS DNA polymerase (Takara Bio, Otsu, Japan) and the primers 5-CGATAACATATGATGATTCGAGTTGCGATTG-3 (forward) and 5-CGATAACTCGAGTTATAAAATATTTTCTAG-3 (reverse)

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Clinical studies in obese, insulin-resistant patients further support this effect, showing that VLCKD increased Bacteroides and decreased Firmicutes [169, 171]

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For optimal GH pulse studies, researchers commonly administer the blend prior to sleep, aligning with the bodys natural nocturnal GH secretion cycle